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A lab tip for you! I've recently decided to reduce contamination of any kind. The only way that I can resolve that problem is to aliquot anything and everything! When it comes to my work with RNA, I need to use RNase and DNase free water so after opening the rather large jug I made around 250 1 ml aliquots and froze it down in our -80 degree Celsius freezer. That way, it is the same water making experiments consistent and prevents double dipping leading to contamination or screwy results. So it comes down to taking the time to do the right thing and not be lazy. One of the hardest things to do, in my opinion!
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